Genomic organization of PDE genes. Panel A: Southern blot analysis. Genomic DNA of L. major was digested with BspHI (lane 1), BspHI plus ScaI (lane 2), BspHI plus NruI (lane 3), BspHI plus AvrII (lane 4), NotI (lane 5), NotI plus BglII (lane 6), NotI plus AvrII (lane 7), NotI plus ScaI (lane 8), AseI (lane 9) and SphI (lane 10). The blots was hybridized with probes B1 and B2 that specifically recognize LmjPDEB1 or LmjPDEB2, respectively (see panel B), or with probe "B" that hybridizes to both genes. The sizes of the DNA size markers are given on the left. Panel B: Organization of the LmPDEB locus on chromosome 15. Restriction sites used in the mapping are indicated. The uncharacterized stretch between the two coding regions is estimated to be about 5 kb long. Filled boxes labelled B1, B2 and "B" indicate the gene regions used as hybridization probes. Panel C: Genomic organization of the LmjPDEB1/LmjPDEB2 locus on chromosome 15 of L. major. The organization of the corresponding loci of T. brucei and T. cruzi are given for comparison. T. brucei: TbrPDEB1 (old designation: TbPDE2C; Tb09.160.3590) and TbrPBEB2 (old designation TbPDE2B; Tb09.160.3630) and NHP/2RS6 (Tb09.160.3670). T. cruzi: TcrPDEB1 (Tc00.1047053508277.100) followed by TcrPDEB2 (old designation TbPDE1; Tc00.104053508277.120; GenBank accession number AAP49573) and by NHP2/RS6 (Tc00.104053508277.120). Numbers indicated nucleotide positions on the respective chromosomes.