Table 4 Bacterial strains and plasmids used in this study.

E. coli

DH5α

φ80dlacZ ΔM15 (lacZYA-argF) recA1 endA gyrA96 thi -1 hsdR17 supE44 relA1 deoR U169

Invitrogen

SM10

Mobilizing strain, RP4 tra genes integrated in the chromosome, Km^r

[50]

DH10B

F^-mcrA Δ(mrr-hds RMS-mcr BC) φ80dlac ZΔM15 Δlac X74 end A1 rec A1 deo R Δ(ara, leu)7697 ara D139 gal U gal K nup G rps L λ-

Invitrogen

JM109 F'

end A1 rec A1 gyr A96 thi hsd R17 (r_k- m_k+) rel A1 sup E44 Δ(lac-pro AB) [F' tra D36 pro AB laq IqZΔM15]

Promega

B. cenocepacia

K56-2

Cystic fibrosis respiratory isolate

[51]

K56-R2

cepR:: Tn5-OT182 derivative of K56-2, Tc^r

[11]

K56-I2

cepI::tp derivative of K56-2, Tp^r

[11]

K56-dI2

cepI deletion mutant of K56-2

[35]

CLW101

cepI::tp::Tn5-OT182 derivative of K56-2, Tc^r, Tp^r

[20]

K56-I2-P1

BCAM03092:: Tn5-OT182 derivative of K56-I2, Tc^r

This study

K56-I2-P3

BCAM0957:: Tn5-OT182 derivative of K56-I2, Tc^r

This study

K56-I2-P5

BCAS0293:: Tn5-OT182 derivative of K56-I2, Tc^r

This study

K56-I2-P9

BCAM2631:: Tn5-OT182 derivative of K56-I2, Tc^r

This study

K56-I2-P12

BCAM2630:: Tn5-OT182 derivative of K56-I2, Tc^r

This study

K56-I2-2PB2

Tn5-OT182 derivative of K56-I2, Tc^r

This study

K56-I2-NB12

BCAM1187:: Tn5-OT182 derivative of K56-I2, Tc^r

This study

Plasmids

pCR^®2.1 TOPO

PCR cloning vector, pUC ori, Plac, lacZα, Km^R Ap^R

Invitrogen

pOT182

pSUP102(GM)::Tn5-OT182, Cm^r, Tc^r, Gm^r, Ap^r

[40]

pALTER^®-Ex 1

mutagenesis plasmid, Tc^r

Promega

pSLS225

pUCP26 with 1.5 kb Sph I-Kpn I fragment containing the cepI gene, Tc^r

[11]

pCPI101

pCR^®2.1 TOPO with a 266 bp fragment containing the cepI promoter, Ap^r, Km^r

This study

pCPI201

pAlter^®-Ex 1 with the Bam HI-Xba I fragment from pCPI101, Tc^r

This study

pMS402

Broad host range vector with promoterless luxCDABE operon, Tp^r, Km^r

[29]

pCPI301

pMS402 with the Bam HI-Xho I fragment containing the wild type cepI promoter region from pCPI101, Tp^r, Km^r

This study

pCPI303-313

pMS402 containing the Bam HI-Xho I fragments containing the cepI promoter region with the cep box mutations designated 303-313, Tp^r, Km^r

This study

pRK2013

ColE1 Tra (RK2)⁺, Km^r

[52]

pPHU301

pMS402 containing the phuR promoter region

This study

pAYL301

pMS402 containing the acyltransferase promoter region

This study

pSCP301

pMS402 containing the scpB promoter region

This study

pAID301

pMS402 containing the aidA promoter region

This study

pMST005

pMS402 containing the MST005 promoter region

This study

pMST011

pMS402 containing the MST011 promoter region

This study

pMST028

pMS402 containing the MST028 promoter region

This study

pMST052

pMS402 containing the MST052 promoter region

This study

pMST059

pMS402 containing the MST059 promoter region

This study

pMST068

pMS402 containing the MST068 promoter region

This study

pMST112

pMS402 containing the MST112 promoter region

This study