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Table 3 Sensitivity of real-time PCR for detection of E. sakazakii in broth culture

From: A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae

E. sakazakii concentration

Strain ATCC 29544

Strain ATCC 51329

CFU/ml

CFU/PCR

C T value (target)

C T value (IAC)

C T value

C T value (IAC)

3.6 107

1.8 106

18.52 ± 0.07

-

21.90 ± 0.43

-

3.6 106

1.8 105

22.86 ± 0.30

-

26.46 ± 0.56

-

3.6 105

1.8 104

27.49 ± 0.32

-

31.83 ± 0.62

-

3.6 104

1.8 103

31.76 ± 0.15

-

37.12 ± 0.46

> 42

3.6 103

1.8 102

36.19 ± 0.39

37.90 ± 1.51

41.89 ± 1.01

40.51 ± 0.97

3.6 102

18

39.55 ± 1.15

38.12 ± 1.16

> 44.85

39.08 ± 1.62

36

1.8

42.46 ± 0.54

38.10 ± 1.92

-

39.36 ± 1.10

  1. C T values are means ± standard deviation obtained for two independent DNA extractions with two amplification repetitions per extraction
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BMC Microbiology

ISSN: 1471-2180

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