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Table 3 Sensitivity of real-time PCR for detection of E. sakazakii in broth culture

From: A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae

E. sakazakii concentration Strain ATCC 29544 Strain ATCC 51329
CFU/ml CFU/PCR C T value (target) C T value (IAC) C T value C T value (IAC)
3.6 107 1.8 106 18.52 ± 0.07 - 21.90 ± 0.43 -
3.6 106 1.8 105 22.86 ± 0.30 - 26.46 ± 0.56 -
3.6 105 1.8 104 27.49 ± 0.32 - 31.83 ± 0.62 -
3.6 104 1.8 103 31.76 ± 0.15 - 37.12 ± 0.46 > 42
3.6 103 1.8 102 36.19 ± 0.39 37.90 ± 1.51 41.89 ± 1.01 40.51 ± 0.97
3.6 102 18 39.55 ± 1.15 38.12 ± 1.16 > 44.85 39.08 ± 1.62
36 1.8 42.46 ± 0.54 38.10 ± 1.92 - 39.36 ± 1.10
  1. C T values are means ± standard deviation obtained for two independent DNA extractions with two amplification repetitions per extraction