Figure 2From: Cytokine responses of bovine macrophages to diverse clinical Mycobacterium avium subspecies paratuberculosis strainsIL-10 protein secreted by MDM cells exposed to MAP over time was measured by ELISA. Total amount of protein (pg/ml) is plotted on Y-axis (note that the Y1-axis scales have been optimized for magnitude of IL-10 expression for each strain). Intracellular bacterial numbers were calculated based on genome size of MAP and represented as genome equivalents (GE) on second Y-axis. MDMs stimulated with B1018 and Hu6 MAP isolates gradually up regulated IL-10 secretion from 2-hr until 96-hr PI.Back to article page