Table 3 DNA dilutions and mixtures used in the sensitivity test. The relative abundance of G. abietina DNA to the other genomic background DNA is indicated in parentheses.
From: Specific and sensitive detection of the conifer pathogen Gremmeniella abietina by nested PCR
Test 1G. abietina | Test 2 G. abietina + P. contorta | Test 3 G. abietina + 7 fungi mix | |
|---|---|---|---|
Sample | |||
1 | 5 × 10-1 ng/μl | 5 × 10-1 ng/μl + 4 ng/μl (1:8) | 5 × 10-1 ng/μl + 6 ng/μl (1:12) |
2 | 5 × 10-2 ng/μl | 5 × 10-2 ng/μl + 4 ng/μl (1:80) | 5 × 10-2 ng/μl + 6 ng/μl (1:120) |
3 | 5 × 10-3 ng/μl | 5 × 10-3 ng/μl + 4 ng/μl (1:800) | 5 × 10-3 ng/μl + 6 ng/μl (1:1200) |
4 | 5 × 10-4 ng/μl | 5 × 10-4 ng/μl + 4 ng/μl (1:8000) | 5 × 10-4 ng/μl + 6 ng/μl (1:12000) |
5 | 5 × 10-5 ng/μl | 5 × 10-5 ng/μl + 4 ng/μl (1:80000) | 5 × 10-5 ng/μl + 6 ng/μl (1:120000) |
6 | 5 × 10-6 ng/μl | 5 × 10-6 ng/μl + 4 ng/μl (1:800000) | 5 × 10-6 ng/μl + 6 ng/μl (1:1200000) |
7 | 5 × 10-7 ng/μl | 5 × 10-7 ng/μl + 4 ng/μl (1:8000000) | 5 × 10-7 ng/μl + 6 ng/μl (1:12000000) |
3 μl in PCR | 1:1 vol. mix, 3 μl in PCR | 1:1 vol. mix, 3 μl in PCR |