Table 2 Protein features found to be discriminative for identification of non-classically secreted Gram-positive (a) and Gram-negative (b) bacterial proteins

Threonine contents

Composition

see Materials & Methods

Transmembrane helices

TMHMM 2.0

[55]

Gravy

ExPASy, ProtParam

[56]

Protein disorder

DisEMBL

[41]

Secondary structure

PSIPRED

[57]

Feature

Program

Reference

Arginine contents

Composition

see Materials & Methods

Instability index

ExPASy, ProtParam

Protein disorder

DisEMBL

[41]