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Figure 6 | BMC Microbiology

Figure 6

From: A gonococcal homologue of meningococcal γ-glutamyl transpeptidase gene is a new type of bacterial pseudogene that is transcriptionally active but phenotypically silent

Figure 6

Western blotting with anti-meningococcal GGT rabbit antiserum [25] (A) and Coomassie Brilliant Blue staining (B) of the whole cell extracts after SDS-PAGE. Bacterial whole cell extracts equivalent to 0.025 OD600 were analyzed. Lane 1, N. meningitidis strain H44/76; lane 2, HT1089 (H44/76 Δggt::spc); lane 3, ATCC49226 (N. gonorrhoeae); lane 4, NIID54 (N. gonorrhoeae); lane 5, NIID103 (N. gonorrhoeae); lane 6, HT1195 (NIID103 Δggh::spc); lane 7, NIID106 (N. gonorrhoeae); lane 8, HT1196 (NIID106 Δggh::spc). Black arrows show the bands corresponding to the processed small and large subunits of meningococcal GGT and the gray arrow indicates the 15-kDa band corresponding to the truncated protein product of the gonococcal ggh gene. M stands for molecular weight marker.

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