Figure 2
From: Immunoreactivity of the Mycobacterium avium subsp. paratuberculosis 19-kDa lipoprotein
SDS-PAGE and immunoblot analysis of the MBP-19 kDa fusion protein. (A) An SDS-PAGE gel showing the noninduced and IPTG-induced E. coli protein lysates. Lanes: 1- protein size markers; 2- noninduced E. coli MBP-19 kDa; 3- IPTG induced E. coli MBP-19 kDa; 4- Affinity purified MBP-19 kDa. (B) Immunoblot probed with a monoclonal antibody to MBP. Lane 1- purified MBP; Lane 2- noninduced E. coli MBP-19 kDa; Lane 3- IPTG induced E. coli MBP-19 kDa.