Table 1 Primers used to evaluate the expression of mRNA transcripts by RT-PCR.
Gene | GenBank Accession No. | Â | Primer 5'-3' sequence |
|---|---|---|---|
beta-actin | NM001101 | Fwd | ATCTGGCACCACACCTTCTACAATGAGCTGCG |
| Â | Â | Rvs | CGTCATACTCCTGCTTGCTGATCCACATCTGC |
CD4 | BC025782 | Fwd | GTGAACCTGGTGGTGATGAGAGC |
| Â | Â | Rvs | GGGGCTACATGTCTTCTGAAACCGGTG |
CXCR-4 | BC020968 | Fwd | CTGAGAAGCATGACGGACAAGTACAG |
| Â | Â | Rvs | CAACAGCTTCCTTGGCCTCTGACT |
CCR-1 | NM001295 | Fwd | GGAAACTCCAAACACCACAGAGGA |
| Â | Â | Rvs | AAGATCTCGCTGTACAAGCCTGTG |
CCR-2 | NM000647 | Fwd | CTCTCCCATTGTGGGCTCACTCTG |
| Â | Â | Rvs | GCAAACACAGCATGGACAATAGCC |
CCR-3 | AF026535 | Fwd | CTATGATGACGTGGGCCTGCTC |
| Â | Â | Rvs | AAGATCTCGCTGTACAAGCCTGTG |
CCR-4 | NM005508 | Fwd | CACCAAAGAAGGCATCAAGGCAT |
| Â | Â | Rvs | TGCCACTGTAAAAGCCCACCAAG |
CCR-5 | NM000579 | Fwd | CCTGATAAACTGCAAAAGGCTGAAG |
| Â | Â | Rvs | AGCAAACACAGCATGGACGACAG |
IL-6 | NM000600 | Fwd | ATGAACTCCTTCTCCACAAGCGC |
| Â | Â | Rvs | GAAGAGCCCTCAGGCTGGACTG |
IL-6R | X12830 | Fwd | CATTGCCATTGTTCTGAGGTTC |
| Â | Â | Rvs | AGTAGTCTGTATTGCTGATGTC |
IL-8 | NM000584 | Fwd | ATGACTTCCAAGCTGGCCGTGGCT |
| Â | Â | Rvs | TCTCAGCCCTCTTCAAAAACTTCTC |
TGF-beta 1 | NM000660 | Fwd | GCCCTGGACACCAACTATTGCT |
| Â | Â | Rvs | GGACGGGGATGTAAACCTCGGA |
TGF-beta 2 | M19154 | Fwd | GATTTCCATCTACAAGACCACGAGGGACTTGC |
| Â | Â | Rvs | GCTTACCGAGAGGAAGCTACATTGACTACGAC |
CPFS3 | AF171877 | Fwd | AATGGCTGGCAAACCCTTCTAATG |
| Â | Â | Rvs | CATCGTCTTCACTTCCCTCTTCACA |