Figure 3
Neutralization by anti-Tat antibodies of the proliferative response to Tat of five different human epithelial cell lines cultured in low-serum medium. Cells were either left untreated (open bars) or exposed to 100 ng/ml Tat86aa (closed bars) at time 0. Cells were counted by microscopy at day-0, -1 and -2 after plating. Antibody tMAb-B (1 μg/ml) was mixed with Tat before treatment (red bars) or given alone (green bars). Bars represent the mean of three wells. Experiments on AV-3 cells (bottom panel) have also demonstrated the Tat-neutralizing activity of a rabbit polyclonal anti-Tat antibody: anti-Tat plus Tat (yellow bars) and anti-Tat alone (gray bars). Results are expressed as number of cells/well. Each bar represents the average of 3 different tests. Standard deviations of the mean are not reported, but were within 12% of the mean.