Figure 6

Dose response relationship between bacterial titer and phosphatidylserine exposure in Cpn infected HASMC. Cells infected with Cpn-K6 in doses between 8 – 386 IFU/cell or with Cpn-VR1310 in doses between 1 – 32 IFU/cell were stained with annexin-V / propidium iodide and analyzed by flow cytometry 48 h post infection. Mock inoculated cells are shown as controls. In figure A and B pooled data out of three independent experiments are displayed. A: The amount of single annexin-V positive cells increased dependent on the chlamydial infectious dose (-◆ -: Cpn-K6; -●-: Cpn-VR1310) but not in mock treated (-○-) cells. (*: significant compared to mock treated cells with p < 0.001) B: the number of single annexin-V positive cells increased over the time when cells were infected with Cpn-K6 128 IFU/cell (-●-) but not if they were mock treated (-○-) (significant compared to mock treated cells with p < 0.001)(#)). C: Dot plots of one representative experiment are depicted to illustrate the amount of annexin V / propidium iodide double labeled cells. Upper left panel: HASMC treated with staurosporin (apoptosis). Upper right panel: HASMC treated with Na-azide (necrosis). Middle left panel: HASMC infected with Cpn-VR1310 at 32 IFU / cell 48 h post infection. Middle right panel: HASMC infected with Cpn-K6 at 128 IFU / cell 48 h post infection. Lower left panel: mock treated HASMC.