Table 2 Comparison of three PCR assays and virus isolation from koi carp exposed to KHV

1

+

-

-

+

sick fish with clinical symptoms

2

-

-

-

-

survivor (unknown time lap after exposure).

3

-

-

-

-

survivor (unknown time lap after exposure) from a tank with mortality.

4

+

-

-

+

survivor (unknown time lap after exposure) from a tank with mortality.

5

-

-

-

-

survivor moved to 30°C, then to 24°C. no mortality in the tank.

6

+

-

-

+

survivor moved to 30°C, then moved to 24°C. 25% mortality in the tank.

7

-

-

-

-

naïve fish that lived at 16°C. No mortality in the tank.

8

+

+

+

+

fish from tank with mortality.

9

+

-

-

+

fish from tank with mortality.

10

-

-

-

Pooled

survivor, 2 years after experimental infection

11

-

-

-

-

survivor, 2 years after experimental infection

12

-

-

-

Pooled

survivor, 1 year after experimental infection

13

-

-

-

-

survivor, 1 year after experimental infection

14

-

-

-

Pooled

survivor, 2 months after experimental infection

15

-

-

-

survivor, 2 months after experimental infection

16

+

-

-

+

survivor, 2 months after experimental infection

17

+

-

-

survivor, 2 months after experimental infection

18

+

-

-

+

survivor from tank with disease- two weeks post infection.

KF+

+

+

+

+

KF-1 cell line infected with KHV- Positive control.

KF-

-

-

-

-

KF-1 cell line- Negative control.