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Table 2 Comparison of three PCR assays and virus isolation from koi carp exposed to KHV

From: Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis

Sample TK PCR Gilad PCR Gray PCR Virus Isolation History of the koi carp
1 + - - + sick fish with clinical symptoms
2 - - - - survivor (unknown time lap after exposure).
3 - - - - survivor (unknown time lap after exposure) from a tank with mortality.
4 + - - + survivor (unknown time lap after exposure) from a tank with mortality.
5 - - - - survivor moved to 30°C, then to 24°C. no mortality in the tank.
6 + - - + survivor moved to 30°C, then moved to 24°C. 25% mortality in the tank.
7 - - - - naïve fish that lived at 16°C. No mortality in the tank.
8 + + + + fish from tank with mortality.
9 + - - + fish from tank with mortality.
10 - - - Pooled survivor, 2 years after experimental infection
11 - - - - survivor, 2 years after experimental infection
12 - - - Pooled survivor, 1 year after experimental infection
13 - - - - survivor, 1 year after experimental infection
14 - - - Pooled survivor, 2 months after experimental infection
15 - - -   survivor, 2 months after experimental infection
16 + - - + survivor, 2 months after experimental infection
17 + - -   survivor, 2 months after experimental infection
18 + - - + survivor from tank with disease- two weeks post infection.
KF+ + + + + KF-1 cell line infected with KHV- Positive control.
KF- - - - - KF-1 cell line- Negative control.