Figure 4

Sensitivity of three PCR assays for the diagnosis of KHV infection. A TK based PCR was used to detect KHV DNA and was compared to two other PCR protocols: the Gilad et al. (2002) and Gray et al. (2002). Details of primers, products and protocols are described in table 1. To compare the sensitivities of the assays, 10 fold serial dilutions of purified KHV DNA were prepared starting from 10 ng DNA per PCR reaction. PCR products were analyzed on a 1% agarose gel and visualized by ethidium bromide. For each protocol the highest dilutions that still give products are shown. The arrows denote the 200 and 500 bp. size markers.