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Table 1 Initial Rates of Methylation Group Incorporationa

From: Site-specific and synergistic stimulation of methylation on the bacterial chemotaxis receptor Tsr by serine and CheW

Ligand

Modification Pattern

[Serine], μM

[CheW], μM

EQQQ

QEQQ

QQEQ

QQQE

QQQQ

0

0

1.7 ± 0.1

0.56 ± 0.07

0.41 ± 0.03

11.5 ± 0.27

0.33 ± 0.03

500

0

5.4 ± 1.3

1.2 ± 0.2

0.50 ± 0.03

13.2 ± 1.6

0.36 ± 0.01

0

60

11.2 ± 1.2

3.4 ± 0.1

2.1 ± 0.1

20.9 ± 1.6

0.69 ± 0.04

500

60

28.4 ± 3.1

8.9 ± 1.6

4.4 ± 0.2

24.6 ± 3.7

0.59 ± 0.02

  1. aThe average of two experiments (± std. error) in millimoles of methyl group per mole of CheR per second.
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BMC Microbiology

ISSN: 1471-2180

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