Table 1 Plasmids and strains
From: Doxycycline-regulated gene expression in the opportunistic fungal pathogen Aspergillus fumigatus
Plasmids | Selectable marker | Transactivator | ||||
|---|---|---|---|---|---|---|
| Â | Promoter | Gene | Terminator | Promoter | Gene | Terminator |
p473 | - | - | - | gpdA(-679) | tTA | cgrA |
p434 | gpdA(-679) | hph | trpC | gpdA(-679) | tTA | cgrA |
p444 | gpdA(-679) | ble | CYC1 | gpdA(-679) | tTA | cgrA |
p474 | - | - | - | gpdA(-679) | rtTA | cgrA |
p502 | gpdA(-679) | hph | trpC | gpdA(-679) | rtTA | cgrA |
p480 | gpdA(-679) | ble | CYC1 | gpdA(-679) | rtTA | cgrA |
p482 | tetO7-gpdA(-175) | hph | trpC | - | - | - |
p500 | TcgrA-tetO7-PgpdA(-175) | hph | trpC | - | - | - |
Strains | Genotype/construction | Source | ||||
wt | H237 | David Holden | ||||
Af-tTA-1 | H237 (p444, p482) – isolate 1 | This study | ||||
Af-tTA-2 | H237 (p444, p482) – isolate 2 | This study | ||||
Af-rtTA | H237 (p480, p500) | This study | ||||