Allele specific synthetic lethality between priC and dnaAts alleles at the permissive temperature of 30°C in E. coli K-12

Table 2 P1 crosses using dnaA^ts strains as the donors and isogenic priC303::kan and dnaC mutants as the recipients^a

Donor		Recipient Strain Number	Recipient Genotype		Ts/total
Strain Number	dnaA		priC	dnaC
JC12390	508	JC13509	+	+	49/53
AL0454	46	JC13509	+	+	60/69
JC12390	508	SS145	303	+	0/72
AL0454	46	SS145	303	+	0/72
SS1750	5	SS145	303	+	7/12
SS1751	167	SS145	303	+	11/11
WM433	204	SS145	303	+	4/5
SS1752	602	SS145	303	+	10/12
JC12390	508	SS1099	303	809	0/63
JC12390	508	SS1100	303	809,820	41/48
AL0454	46	SS1099	303	809	0/32
AL0454	46	SS1100	303	809,820	0/24

^aThese are the results of either single or multiple transductions. The numbers show the number of Tet^R transductants that were also sensitive to growth at 42°C. If the transductions yielded temperature sensitive transductants then only a few transductants from one transduction is reported. The recipient cells were grown at 37°C in Luria broth. They were then treated with donor lysate made on the tnaA300:: Tn10 dnaA^ts strain indicated. The transductions were then spread on Luria plates containing 10 μgm/ml tetracycline and incubated 24–48 hours at 30°C. Transductants were then picked, purified and tested for growth at 42°C by replica plating.

ISSN: 1471-2180