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Table 2 P1 crosses using dnaAts strains as the donors and isogenic priC303::kan and dnaC mutants as the recipientsa

From: Allele specific synthetic lethality between priC and dnaAts alleles at the permissive temperature of 30°C in E. coli K-12

Donor Recipient Strain Number Recipient Genotype Ts/total
Strain Number dnaA   priC dnaC  
JC12390 508 JC13509 + + 49/53
AL0454 46 JC13509 + + 60/69
JC12390 508 SS145 303 + 0/72
AL0454 46 SS145 303 + 0/72
SS1750 5 SS145 303 + 7/12
SS1751 167 SS145 303 + 11/11
WM433 204 SS145 303 + 4/5
SS1752 602 SS145 303 + 10/12
JC12390 508 SS1099 303 809 0/63
JC12390 508 SS1100 303 809,820 41/48
AL0454 46 SS1099 303 809 0/32
AL0454 46 SS1100 303 809,820 0/24
  1. aThese are the results of either single or multiple transductions. The numbers show the number of TetR transductants that were also sensitive to growth at 42°C. If the transductions yielded temperature sensitive transductants then only a few transductants from one transduction is reported. The recipient cells were grown at 37°C in Luria broth. They were then treated with donor lysate made on the tnaA300:: Tn10 dnaAts strain indicated. The transductions were then spread on Luria plates containing 10 μgm/ml tetracycline and incubated 24–48 hours at 30°C. Transductants were then picked, purified and tested for growth at 42°C by replica plating.