The lps locus is absent from the genomes of Xoo strains BXO8, Nepal624 and Xoor strain BXORI. (A) PCR analysis using primers that are specific to wxoA gene. M is the λ Hind III Marker lane. An expected band of 1 kb (indicated by arrow) is present in the Xoo strains, BXO1 (lane 1), BXO5 (lane 3), BXO6 (lane 4) and BXO20 (lane 6) but absent in BXO8 (lane 5), Nepal624 (lane 7) and BXORI (lane 2). (B) Southern hybridization analysis of Eco RI digested genomic DNA using α-32-P labeled wxoA specific probe (see Methods). A 4 kb band can be seen (indicated by arrow) in BXO1 (lane 1) but not in BXORI (lane 2), BXO8 (lane 3) and Nepal624 (lane 4). Similar results were obtained for wxoB, wxoC, wxoD, wzm and wzt genes. (C) The blot from (B) was deprobed and was hybridized with α-32P labeled probe specific to the metB gene. A specific band can be seen in all the lanes. Note the sizes of the bands indicating that metB is present in different Eco RI fragments in BXO1, BXORI and BXO8/Nepal624.