Sequence alignment of the probe region of M. hominis isolates. The sequence of PG21 was used as a template. The probe sequences from the M. hominis isolates are shown. Highlighted base pairs corresponded to differences in probe sequences in comparison to PG21 standard. Groups are shown in boxes: top box represents the lowest melting temperature of 62°C, group 3, with isolates; 3105, 4712 and 5941; medium box contains: 7808, DC63, P2, SC4, 132, 7357 and 93 that have a melting temperature of 64°C; and bottom box with V2785 is almost identical to PG21 with only one mismatch and a melting temperature of 66°C.