Specificty of the LightCycler PCR. (a) LightCycler PCR with human DNA from Hep2 cells and selected Mycoplasma spp. run with the designed primers and probes. (b) Spiking assay showed no inhibition when DNA from five different Mycoplasma species (M. arginini, M. bovis, M. hyorhinis, M. pulmonis, M. salivarium) was spiked with PG21 DNA of 102 copies/μl. All curves came up at the same time as 102 copies/μl of PG21 from the standard dilution series (marked with squares).