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Table 1 Optimal m.o.i. values using RRV C6/36 stock virus with respective HA titre and hours of incubation in C6/36, PS-EK, Vero and BHK-21 C13 cell lines

From: Sucrose density gradient centrifugation and cross-flow filtration methods for the production of arbovirus antigens inactivated by binary ethylenimine

Cell Line m.o.i. (PFU/cell) Maximum HA Titre at pH 5.8 Hours of Incubation
C6/36 1.5 × 10-4 1024 51
  1.5 × 10-5 1024 51
PS-EK 1.1 × 10-1 1024 47
  4.4 × 10-2 1024 47
  2.2 × 10-2 1024 51
Vero 7.5 × 10-2 8192 47
  3.0 × 10-2 8192 47
  1.5 × 10-2 8192 47
  1.5 × 10-3 8192 47
  1.5 × 10-4 8192 47
BHK-21 C13 7.9 × 10-2 8192 51
  3.2 × 10-2 8192 47
  1.6 × 10-2 8192 47
  1.6 × 10-3 8192 51