Table 1 Optimal m.o.i. values using RRV C6/36 stock virus with respective HA titre and hours of incubation in C6/36, PS-EK, Vero and BHK-21 C13 cell lines
Cell Line | m.o.i. (PFU/cell) | Maximum HA Titre at pH 5.8 | Hours of Incubation |
|---|---|---|---|
C6/36 | 1.5 × 10-4 | 1024 | 51 |
|  | 1.5 × 10-5 | 1024 | 51 |
PS-EK | 1.1 × 10-1 | 1024 | 47 |
|  | 4.4 × 10-2 | 1024 | 47 |
|  | 2.2 × 10-2 | 1024 | 51 |
Vero | 7.5 × 10-2 | 8192 | 47 |
|  | 3.0 × 10-2 | 8192 | 47 |
|  | 1.5 × 10-2 | 8192 | 47 |
|  | 1.5 × 10-3 | 8192 | 47 |
|  | 1.5 × 10-4 | 8192 | 47 |
BHK-21 C13 | 7.9 × 10-2 | 8192 | 51 |
|  | 3.2 × 10-2 | 8192 | 47 |
|  | 1.6 × 10-2 | 8192 | 47 |
|  | 1.6 × 10-3 | 8192 | 51 |