Activation of the HCE cell NF-κB by RSV. (A) Amounts of p65 and p50 subunits of NF-κB were determined by immunoblot of isolated nuclei. Note that a control transcription factor, Sp1, was not activated . (B) Functional activation of NF-κB in RSV-infected cells was measured by luciferase induction from a reporter plasmid (pNFκB-Luc) as described in Methods. Where indicated, 10 or 20 mM NAC, Na-salicylate (SAL) or aspirin (ASP) was included in the reaction. Each bar is derived from three independent experiments with the standard error as indicated.