Figure 1

Colony PCR reactions with primers specific for the omp35 gene or the chloramphenicol acetyltransferase gene ( cat ) from pEP185.2. Lanes 1 and 2 are reactions with omp35 primers O1 and O2 (see Table 2) and lanes 3–5 are reactions with cat primers C1 and C2 (see Table 2). The templates for PCR were as follows: lanes 1 and 3, MR-1; lanes 2 and 4, OMP35-1; lane 5, pDSEPomp35. The sizes of the DNA markers (lane M) in kilobases are indicated on the left.