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Table 1 Bacterial strains used in this study

From: Expression of a novel gene, gluP, is essential for normal Bacillus subtilis cell division and contributes to glucose export

Strain Relevant genotype/phenotype Reference/source
B. subtilis   
168 trpC2 (wild type) BGSCa, 1A1
MD153 trpC2 ptsG::cat [40]
MD186 trpC2 glcK::spc [9]
MD189 trpC2 glcK::spc ptsGHI::erm [9]
MD191 trpC2 glcK::spc ptsG::cat [9]
MD195 trpC2 amyE::(cat-lacZ) pAC6 tf> 168b
MD196 trpC2 amyE::(2112 bp Eco RI-Bam HI fragment-lacZ, cat) pLR-P01 tf> 168b
MD197 trpC2 amyE::(1994 bp Eco RI-Bam HI fragment-lacZ, cat) pLR-P011 tf> 168b
MD198 trpC2 amyE::(1673 bp Eco RI-Bam HI fragment-lacZ, cat) pLR-P012 tf> 168b
MD199 trpC2 amyE::(974 bp Eco RI-Bam HI fragment-lacZ, cat) pLR-P013 tf> 168b
MD202 trpC2 amyE::(659 bp Eco RI-Bam HI fragment-lacZ, cat) pLR-P014 tf> 168b
MD204 trpC2 amyE::(464 bp Eco RI-Bam HI fragment-lacZ, cat) pLR-P021 tf> 168b
MD205 trpC2 amyE::(1163 bp Eco RI-Bam HI fragment-lacZ, cat) pLR-P031 tf> 168b
MD206 trpC2 amyE::(1046 bp Eco RI-Bam HI fragment-lacZ, cat) pLR-P032 tf> 168b
MD207 trpC2 amyE::(1308 bp Eco RI-Bam HI fragment-lacZ, cat) pLR-P015 tf> 168b
MD229 trpC2 gluP::aphA3 pLM-YqgP-Km1 tf> 168b
MD230 trpC2 glcK::spc gluP::aphA3 pLM-YqgP-Km1 tf> MD186b
MD231 trpC2 glcK::spc ptsGHI::erm gluP::aphA3 pLM-YqgP-Km1 tf> MD189b
MD232 trpC2 ptsG::cat gluP::aphA3 pLM-YqgP-Km1 tf> MD153b
MD233 trpC2 glcK::spc ptsG::cat gluP::aphA3 pLM-YqgP-Km1 tf> MD191b
MD234 trpC2 ptsGHI::erm gluP::aphA3 Chr. DNA QB6020 tf> MD229b
QB6020 trpC2 ptsGHI::erm [51]
E. coli   
TG1 supE hsd 5 thi (lac-proAB) F'(traD36 proAB+ lacIq lacZ M15 [52]
  1. aBGSC, Bacillus Genetic Stock Center, Ohio State University, Columbus, Ohio btf> denotes transformation of linear plasmid DNA in indicated strain.