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Figure 5 | BMC Microbiology

Figure 5

From: Type III secretion proteins PcrV and PcrG from Pseudomonas aeruginosa form a 1:1 complex through high affinity interactions

Figure 5

PcrV binding to PcrG and PcrG (1–74) demonstrated by surface plasmon resonance. Injection of PcrV (2–180 nM, flow rate: 40 μl/min, injected volume: 160 μl) over (A) immobilized PcrG (213 RU); (B) immobilized PcrG (1–74) (548 RU). Non-specific binding to the sensor chip (evaluated on flow cell 1) and the bulk effect due to the buffer were subtracted from the raw data.

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