Susceptibility of chaperone- or protease-deficient mutants of E. coli to LVFX. Bacterial cultures were diluted from 10-3 to 10-7 and then spotted on to agar plates prepared by the standard agar doubling dilution method. (A) and (C) are the results with drug-free agar plates. (B) and (D) are the results with agar plates containing 0.0125 μg/ml LVFX. Abbreviation: plon+, a complementing plasmid carrying the intact lon gene .