Figure 3

Analysis of allele variability by agarose gel electrophoresis and fluorescent tagged capillary electrophoresis. [A] Amplified fragments from three representative VNTR loci were resolved by electrophoresis in a horizontal gel composed of 3% Metaphor agarose. Sample identifications are given above their respective lanes. Calculated alleles (repeat units) are provided in red under each product. Product sizes range from 143–193 bp (Locus 1); 91–157 bp (Locus-7); and 86 bp (Locus-8). [B] Electropherograms generated from the capillary electrophoresis of fluorescently tagged products amplified from VNTR Locus-1 of the same strains assayed in Panel A. Product sizes (in bp) are shown in boxes below the major peaks. Sizes were calculated by the GeneScan software relative to the GeneScan 500-ROX size markers included in each sample as an internal standard. The vertical axis is the relative peak height of the detected fluorescent products. The first major peak in each electropherogram is the run-through of unincorporated primer.