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Table 2 The inhibition of methionine adenosyltransferase by purine analogues. The M. smegmatis enzyme was incubated with 1 mM ATP, 10 mM methionine, 20 mM Mg2+, 150 mM K+, and 10 mM inhibitor, as described in the Methods section.

From: Characterisation of methionine adenosyltransferase from Mycobacterium smegmatis and M. tuberculosis

Inhibitor Inhibition (%) Inhibitor Inhibition (%)
8-Chlorotheophylline 7.0 ± 4.1 1,3,7-Trimethyluric acid 0.0 ± 0.0
7-Hydroxypropyl theophylline 16.1 ± 4.0 6-Bromopurine 31.4 ± 1.5
Uric acid 45.6 ± 11.9 7-Methylxanthine 36.3 ± 4.2
Xanthine 35.4 ± 2.5 1-Methylxanthine 35.9 ± 5.8
8-Azaguanine 81.7 ± 2.8 2-Hydroxypurine 33.8 ± 0.6
3,7-Dimethyluric acid 27.9 ± 1.6 6-Chloropurine 31.4 ± 7.3
2,6-Dichloropurine 35.5 ± 6.9 2-Amino-6-chloropurine-9-acetic acid 23.5 ± 8.4
6-Mercaptopurine 40.1 ± 2.3 6-Benzyloxypurine 17.7 ± 3.4
1-Methyluric acid 43.4 ± 2.9 2-Aminopurine 11.0 ± 2.1
Purine riboside 44.4 ± 5.9 6-Methylpurine 0.0 ± 0.0
O-Methylguanine 60.3 ± 5.3 7-Methyluric acid 11.4 ± 2.2
2,6-Diaminopurine 29.3 ± 7.9 6-Cyanopurine 24.2 ± 4.6
2-Amino-6-carboxy ethyl mercaptopurine 31.9 ± 9.8 2-Amino-6-chloropurine riboside 17.2 ± 3.3
6-Propoxypurine 27.9 ± 4.0 6-Dimethyl aminopurine 28.0 ± 5.3
6-Dimethylallyl aminopurine riboside 41.6 ± 5.5 Azathioprine 75.5 ± 5.4
8-Aza-2,6-diaminopurine 40.0 ± 2.2 6-Mercaptopurine riboside 30.0 ± 5.7
6-Chloropurine riboside 17.1 ± 3.5