Time dependent expression of Pmps Histogram showing the contribution of selected Pmps to the total amount of protein synthesized in a two-hour labeling period. Chlamydia proteins were selectively labeled with [35S]methionine under the addition of cycloheximide to stop host cell protein synthesis, separated by 2-D PAGE and visualized by autoradiography. Infected cells were labeled at 24, 36 and 48 hours post infection. Spots were detected by using the MelanieII software. Spot volumes were calculated as density integrated over Gaussian area, and the percentage of total spot volume in each gel was calculated. The percentages were corrected for the methionine content of each protein. The corrected spot volume percentages are given in arbitrary units at the Y-axis. Only Pmps located in the gel at positions where no overlapping protein spots hindered quantification are depicted. MOMP and DnaK are included as controls.