Figure 3

Protease protection assays Lane 1 – undigested SecB, lane 2 – SecB digested with proteinase K; lanes 3 through 10 – SecB was incubated with potential substrates at the indicated concentrations prior to proteinase K digestion; lane 3 – 20 nM poly-L-lysine, lane 4 – 150 nM poly-L-lysine; lane 5 – 150 nM N^102LT (wild type), lane 6 – 4 μM N^102LT; lane 7 – 150 nM LA57-N^102LT (slow folding mutant), lane 8 – 4 μM LA57-N^102LT; lane 9 – 150 nM proOmpA, lane 10 – 1.4 μM proOmpA; lane 11 – proOmpA alone digested with proteinase K. Lower molecular weight band in lanes 6 and 8 is the λ repressor fragment.