Figure 1From: Recognition of secretory proteins in Escherichia coli requires signals in addition to the signal sequence and slow foldingImmunodetection of λ repressor fragments in bacterial fractions LA57 indicates the slow folding λ repressor, LA57-N102LT, while wt refers to the wild type λ repressor, N102LT. The strains used were either wild type secY/prlA or prlA4 as indicated. The individual lanes are as follows: M – purified N102LT used as a molecular weight marker, W – whole cell lysate, S – spheroplast fraction (containing cytoplasmic contents and membranes), P – periplasmic fraction.Back to article page