Figure 3
From: Detection and characterization of the S. typhimurium HilA protein
Effect of shifts in aeration conditions on growth, SPI1 gene expression and HilA levels CL87 (iagB87::lacZY) (circles) and EE656 (prgH020::Tn5lacZY) (squares) were grown for 3.5 hours in high osmolarity, low aeration conditions and then shifted to high aeration (open symbols) and low aeration (closed symbols) growth conditions for an additional 0.5, 1.5, 2.5 hours. A. Growth curves. OD600s of individual cultures. B. iagB-lacZ and prgH-lacZ expression. Miller Units of individual cultures. C. HilA protein levels. Western blots of protein samples from individual cultures.