Expression of GST-mycosin-1 fusion protein in E. coli . Bacteria were transformed with vector only (pGEX-2T, left panel) or vector expressing GST-mycosin-1 fusion protein (pGEX-P1, right panel). Lysates were prepared before (U) and after (I) induction with 0.1 mM IPTG for 2 hours; recombinant proteins were purified (P) by GST-affinity chromatography. In each case, fractions were resolved by SDS-PAGE (a) and then analyzed by Western blotting (b) using an anti-GST monoclonal antibody as the primary antibody. Molecular weights (in kDa) are indicated on the left.