EPS production and bacterial motility assays in X. citri , the hrp mutants and the hrpB
c strains. (A) Quantification of EPS present in the supernatant fraction of cultures of the different strains. Quadruplicate measurements were made for each strain and an average of all measurements was obtained. Error bars indicate standard deviations. (B) RT-qPCR assay to determine gumD expression of the different stains relative to X. citri. Values are the means of four biological replicates with three technical replicates each. (C) Quantification of bacterial swimming and swarming motility. Results are the average of the motility zones of 16 Petri dishes per strain. Error bars indicate the standard deviation.