Analysis of T3SS gene expression in leaf-associated grown X. citri and survival of X. citri , hrp mutants and hrpB
c cells associated to leaves. (A) RT-qPCR to determine hrpG, hrpX and hrpE expression levels in X. citri grown associated to leaves. Bars indicate the expression levels of the T3SS genes at two days of leaf-associated growth relative to time 0. Values are the means of four biological replicates with three technical replicates each. (B) X. citri, hrp mutants and hrpB
−c strains leaf-associated survival on citrus leaves. Values represent an average of four leaves assayed for each strain. Error bars indicate the standard deviation.