Skip to main content

Table 1 List of primer sequences used for relative quantitative real-time PCR

From: Mycobacterium bovis BCG infection severely delays Trichuris muris expulsion and co-infection suppresses immune responsiveness to both pathogens

Target

Forward

Reverse

HPRT

GACTGTAGATTTTATCAGACT

GTCTGGCCTGTATCCAACACTTC

GPDH

GGTGGCAGAGGCCTTTG

TGCCGATTTAGCATCTCCTT

*18S [30]

GTCTGTGATGCCCTTAGATG

AGCTTATGACCCGCACTTAC

*TGF-β [30]

CCGCAACAACGCCATCTATG

CTCTGCACGGGACAGCAAT

*IFN-γ [31]

AAGTTCTGGGCTTCTCCTCCTG

GCCAGTTCCTCCAGATATCCAAGA

*IL-10 [30]

CTGGACAACATACTGCTAACCG

GGGCATCACTTCTACCAGGTAA

*IL-4 [31]

TCAACCCCCAGCTAGTTGTC

TTCAAGCATGGAGTTTTCCC

GATA3

CTGGAGGAGGAACGCTAATG

GGTTGAAGGAGCTGCTCTTG

Tbet

AGCAAGGACGGCGAATGTT

GGGTGGACATATAAGCGGTTC

*Foxp3 [30]

CACAATATGCGACCCCCTTTC

AACATGCGAGTAAACCAATGGTA

  1. *Primer sequences adapted from reference.
Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us