Experimental design. (A) BALB/c mice were infected i.n. with M. bovis BCG on day 1, followed by i.g. T. muris infection on day 42. Mice were killed on day 63 and the relevant tissues collected for further analysis. (B) BALB/c mice were infected i.g. with T. muris every 10 days starting on day 1. Animals were co-infected i.n. with M. bovis BCG on day 10. Mice were killed on day 52 and the relevant tissues collected. Appropriate single infections and PBS controls were included in parallel for both protocols. Experiments were performed with 5 to 10 animals per group. P values <0.05 were considered statistically significant. (ns = non significant).