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Table 2 Comparison between Las -LAMP and real time PCR assay sensitivity from DNA purified from a Candidatus Liberibacter asiaticus positive plant

From: Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick

Detection method Purified DNA from a Las positive citrus plant
  100 ng 10 ng 1 ng 100 pg 10 pg 1 pg 100 fg
Las-LAMP Gel + + + + + - -
Las-LAMP FLD + + + + + - -
Real time PCR + + + + + - -
  1. For each dilution the Las-LAMP reaction was performed in triplicate. Gel: gel electrophoresis. LFD: lateral flow dipstick. +: Positive reaction. -: Negative reaction. Real time PCR have been scored as positive if amplification could be detected during the reaction time.