Table 2 Bacterial strains and plasmids used in this study

Pseudomonas syringae

pv. glycinea PG4180

Wild type, levan+

R. Mitchell

pv. phaseolicola 1448A

Wild type, levan+

[33]

pv. syringae B728a

Wild type, levan+

[34]

pv. tomato DC3000

Wild type, levan+

D. Cuppels

Pseudomonas syringae pv. glycinea PG4180

PG4180.M6

Sp^r, Gm^r, lscB lscC mutant of PG4180, levan-

[10]

PG4180.M6(pRA3.1)

Sp^r, Gm^r, Tc^r, lscB lscC mutant of PG4180, containing lscA under control of P _lac on 3.1-kb PstI fragment in pRK415

[10]

Escherichia coli

DH5α

supE44 DlacU169 (F80 lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

[31]

Plasmids

pRK2013

Km^r, helper plasmid

[35]

pLB7.2

Ap^r, contains lscB on 7.2-kb EcoRV insert

[10]

pBBR1MCS

Cm^r, broad-host-range cloning vector

[36]

pBBR1MCS-3

Tc^r, broad-host-range cloning vector

[36]

pBBR3-500-lscB

Tc^r, lscB gene with −500-bp upstream sequence in pBBR1MCS-3

[24]

pBBR3(lscA)

Tc^r, lscA gene containing insert from pRA3.1 in PBBR1MCS-3 not under control of P _lac

This study

pBBR3(lscB_UpNA)

Tc^r, fusion of 518-bp upstream region of lscB (including first 48-bp of coding region) and lscA (including start codon and downstream region) in pBBR1MCS-3

This study

pBBR3(lscB_UpA)

Tc^r, fusion construct of 470-bp upstream region of lscB (without N-terminus) and lscA (including start codon and downstream in pBBR1MCS-3

This study

pBBR3(lscA_UpB)

Tc^r, fusion of 550-bp upstream region of lscA and lscB (including start codon and downstream region) in pBBR1MCS-3

This study