Strain | Description | Reference or source |
---|---|---|
Pseudomonas syringae | Â | Â |
pv. glycinea PG4180 | Wild type, levan+ | R. Mitchell |
pv. phaseolicola 1448A | Wild type, levan+ | [33] |
pv. syringae B728a | Wild type, levan+ | [34] |
pv. tomato DC3000 | Wild type, levan+ | D. Cuppels |
Pseudomonas syringae pv. glycinea PG4180 | ||
PG4180.M6 | Spr, Gmr, lscB lscC mutant of PG4180, levan- | [10] |
PG4180.M6(pRA3.1) | Spr, Gmr, Tcr, lscB lscC mutant of PG4180, containing lscA under control of P lac on 3.1-kb PstI fragment in pRK415 | [10] |
Escherichia coli | ||
DH5α | supE44 DlacU169 (F80 lacZDM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | [31] |
Plasmids | ||
pRK2013 | Kmr, helper plasmid | [35] |
pLB7.2 | Apr, contains lscB on 7.2-kb EcoRV insert | [10] |
pBBR1MCS | Cmr, broad-host-range cloning vector | [36] |
pBBR1MCS-3 | Tcr, broad-host-range cloning vector | [36] |
pBBR3-500-lscB | Tcr, lscB gene with −500-bp upstream sequence in pBBR1MCS-3 | [24] |
pBBR3(lscA) | Tcr, lscA gene containing insert from pRA3.1 in PBBR1MCS-3 not under control of P lac | This study |
pBBR3(lscBUpNA) | Tcr, fusion of 518-bp upstream region of lscB (including first 48-bp of coding region) and lscA (including start codon and downstream region) in pBBR1MCS-3 | This study |
pBBR3(lscBUpA) | Tcr, fusion construct of 470-bp upstream region of lscB (without N-terminus) and lscA (including start codon and downstream in pBBR1MCS-3 | This study |
pBBR3(lscAUpB) | Tcr, fusion of 550-bp upstream region of lscA and lscB (including start codon and downstream region) in pBBR1MCS-3 | This study |