Table 1 β-galactosidase activities (units mg -1 ) for bacterial two-hybrid analysis of RbaW interactions with other proteins
Prey | Bait | |||
|---|---|---|---|---|
pT18c-RbaW | pT18c | pT18c-Zipa | ||
pKNT25 | RbaV | 1440.0 ± 299.0 | 101.4 ± 53.7 | NDb |
RpoD | 131.9 ± 18.6 | 165.0 ± 70.6 | ND | |
RpoHI | 212.7 ± 58.5 | 139.9 ± 32.2 | ND | |
σ2637 | 310.7 ± 13.9 | 124.2 ± 22.9 | ND | |
σ699 | 181.7 ± 54.3 | 201.7 ± 72.2 | ND | |
Empty | 147.0 ± 20.6 | 173.6 ± 23.7 | ND | |
pKT25 | RbaV | 129.4 ± 15.9 | 115.8 ± 32.2 | ND |
RpoD | 236.0 ± 60.8 | 132.4 ± 47.1 | ND | |
RpoHI | 161.0 ± 43.4 | 161.0 ± 6.6 | ND | |
σ2637 | 220.5 ± 54.7 | 178.7 ± 28.3 | ND | |
σ699 | 182.3 ± 63.4 | 199.1 ± 80.0 | ND | |
Empty | 130.4 ± 1.7 | 175.6 ± 9.1 | ND | |
|  | KT-Zipa | ND | ND | 7338.9 ± 1300.0 |