The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts

Clark, Clifford G; Chong, Patrick M; McCorrister, Stuart J; Simon, Philippe; Walker, Matthew; Lee, David M; Nguy, Kimberly; Cheng, Keding; Gilmour, Matthew W; Westmacott, Garrett R

doi:10.1186/1471-2180-14-70

Table 2 Proteins regulated with ≥0.6 log ₂ change in isolate 00–2426 (no CJIE1) compared with isolate 00–2425 (with CJIE1, grown on MH) as reference when isolates were grown on MH and MH + SD

From: The CJIE1 prophage of Campylobacter jejuni affects protein expression in growth media with and without bile salts

Protein name^a	GI number	Locus in NCTC 11168	Protein function or region	log₂change 00–2425 MH + SD agar	log₂change 00–2426 MH agar	log₂change 00–2426 MH + SD agar
CJIE1 00–2425 ORF 11	gi\|313116388	Not present	Unknown	0.67 ± 0.40	−2.43 ± 0.70	−2.13 ± 1.27
CJIE1 signal peptidase I associated with PanB	gi\|315124225	Not present	Prophage repressor	0.50 ± 0.20	−3.75 ± 0.64	−3.15 ± 0.78
Hypothetical protein CJJ81176_0110	gi\|121613178	Cj0073c	Unknown	0.83 ± 0.21	0.87 ± 0.99	1.27 ± 0.67
Iron-sulfur cluster binding protein	gi\|121612415	Cj0074c	Unknown	0.57 ± 0.6	0.63 ± 1.01	1.00 ± 0.61
Anaerobic C4-dicarboxylate transporter	gi\|218561769	Cj0088		1.03 ± 1.04	1.13 ± 0.40	2.17 ± 0.38
Hypothetical protein Cj0170	gi\|218561850	Cj0170	Unknown	−0.30 ± 0.10	−2.80 ± 0.90	−2.97 ± 0.31
Hypothetical protein CJJ81176_0447	gi\|121613189	Cj0427	Unknown	0.33 ± 1.46	1.33 ± 1.12	1.07 ± 1.66
Invasion phenotype protein CipA	gi\|157414972	Cj0685c	Sugar transferase	0.70 ± 0.20	−2.87 ± 0.21	−2.43 ± 0.47
Phosphate acetyltransferase Pta	gi\|121613717	Cj0688	Acetate metabolism, regulation	−0.03 ± 0.21	−2.43 ± 0.31	−2.67 ± 0.58
Acetate kinase AckA	gi\|384443065	Cj0689	Acetate metabolism, regulation	−0.33 ± 0.64	−3.37 ± 0.25	−2.70 ± 1.00
Oxidoreductase	gi\|218562461	Cj0833c	Oxidoreductase	−0.10 ± 0.10	−0.96 ± 1.76	−1.10 ± 0.95
Ankyrin repeat-containing periplasmic protein	gi\|218562462	Cj0834c	Mediates protein-protein interactions	−0.67 ± 0.25	−1.20 ± 2.42	−1.60 ± 1.05
Amino acid ABC transporter, ATP-binding protein PEB1	gi\|121612723	Cj0922c	Amino-acid ABC transporter	0.77 ± 0.21	0.63 ± 0.68	1.03 ± 0.45
Aspartate carbamoyltransferase catalytic subunit PyrB	gi\|218562712	Cj1098	Pyrimidine biosynthesis	0.20 ± 0.35	−1.70 ± 0.17	−1.57 ± 0.21
Oligoendopeptidase F PepF	gi\|218562713	Cj1099	Degradation of macromolecules	−0.20 ± 0.10	−1.43 ± 0.32	−1.63 ± 0.23
ATP-dependent DNA helicase UvrD	gi\|384448349	Cj1101	DNA helicase	0.73 ± 0.42	−1.65 ± 0.21*	−1.23 ± 0.47
Hypothetical protein ICDCCJ07001_1249	gi\|315124755	Cj1305	Part of O-linked glycosylation locus	−0.35 ± 0.35*	1.30 ± 1.56*	2.53 ± 2.60
Hypothetical protein Cj1310c	gi\|218562921	Cj1310c	Part of O-linked glycosylation locus	−0.23 ± 0.25	1.33 ± 1.02	0.87 ± 0.81
Motility accessory factor	gi\|407942710	Cj1318	Part of O-linked glycosylation locus	0.03 ± 0.32	0.93 ± 0.98	1.00 ± 0.17
Maf7; hypothetical protein C8J_1258	gi\|157415578	Cj1342c	Motility accessory factor	0.20 ± 0.10	1.57 ± 0.31	1.87 ± 0.31
Sugar transferase	gi\|218563025	Cj1421c	Part of capsule locus	0.50 ± 0.17	0.80 ± 0.66	1.3 ± 0.60
Methyltransferase protein	gi\|218563030	Cj1426c	Part of capsule locus	0.57 ± 0.72	1.13 ± 0.72	1.03 ± 0.65
Hypothetical protein Cj1429c	gi\|218563033	Cj1429c	Capsule biosynthesis	0.33 ± 0.32	−2.50 ± 0.36	−1.80 ± 1.25
Aminotransferase	gi\|218563040	Cj1436c	Part of capsule locus	−0.30 ± 0.30	−1.53 ± 1.26	−2.03 ± 0.25
Hypothetical protein CJJ81176_1657	gi\|121612948	Cj1666c	Probable periplasmic protein	−0.63 ± 0.46	−0.63 ± 0.51	−1.43 ± 0.38

^aProtein names in bold were also detected in previous experiments after growth on MH + 10% sheep blood. Some experiments returned values for the protein in only 2/3 experiments; these are marked with a `*'. Other information was the same as described in the footnote to Table 1.

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ISSN: 1471-2180

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