pZMO7-Derived shuttle vectors for heterologous protein expression and proteomic applications in the ethanol-producing bacterium Zymomonas mobilis

Table 2 Plasmid copy number determination for pZ7C and pZ7-184 in Z. mobilis NCIMB 11163, CU1 Rif2 and ATCC 29191 strains

Z. mobilis host strain and established shuttle vector	Plasmid copy number
NCIMB 11163
pZ7C	1.8 ± 0.2
pZ7-184	1.2 ± 0.2
CU1 Rif2
pZ7C	1.7 ± 0.3
pZ7-184	2.8 ± 0.3
ATCC 29191
pZ7C	25.1 ± 1.4
pZ7-184	21.8 ± 1.6

Quantitative PCR (qPCR) was used to determine shuttle vector copy number determined using primers targeting the chloramphenicol acetyltransferase (cat) gene. Strains were cultivated in RM media containing 100 μg/ml chloramphenicol (Cm) at 30°C for 24 hours.

ISSN: 1471-2180