The antibacterial effect of DETA/NO in combination with miconazole and polymyxin B nonapeptide. The effect of DETA/NO on bacterial viability when combined with miconazole and polymyxin B nonapeptide (PMBN) in ESBL-producing isolate 7 after exposure for 4, 8 and 24 h. Growth is shown as log CFU/ml of treated bacteria or controls (untreated) and compared to the initial starting inoculum. (A) The effects of miconazole and PMBN per se are shown. *p < 0.05 control vs miconazole (B) The effects of DETA/NO alone and in combination with miconazole are shown. ***p < 0.001 DETA/NO vs DETA/NO + miconazole (C) The effects of DETA/NO alone and DETA/NO in combination with miconazole and PMBN are shown. **p <0.01, ***p < 0.001 DETA/NO vs DETA/NO + miconazole + PMBN. All data are expressed as mean ± SEM of three-four independent experiments.