Figure 7

HSP70 colocalized with viral dsRNA in infected MACR-145 cells. MARC-145 cells were mock infected or infected with PRRSV at an MOI of 0.1. Cells were fixed at 24 h.p.i and subjected to IFA to detect dsRNA (red) and HSP70 (green) by using mouse anti-dsRNA (J2) MAb and rabbit anti-HSP70 polyclonal antibody, respectively. Nuclei were stained with Hoechst dye 33258 (blue). The images of cells were acquired with Leica TCS SP5 confocal microscope.