Genomic comparison of R. grahamii and other rhizobia. A) Chromosomal alignment of R. grahamii and other rhizobial chromosomes. Each replicon was split in silico in 10 kbp fragments and aligned by BlastN with R. grahamii CCGE502 chromosome as a reference (internal black circle with size labels). When 70% of identity in each fragment with the reference was found, a color line was used to indicate the conservation in the genomes. The colors used are: blue for R. etli CFN42, green for R. tropici CIAT 899 and red for R. mesoamericanum STM3625. The black circle with peaks represents the G + C content, and the outside internal circle the GC skew of R. grahamii CCGE502 chromosome. Black arrows indicate the location of the genomic island. B) ANI and C) conserved DNA values between replicons of R. grahamii CCGE502 and R. mesoamericanum CCGE501 (blue) or STM3625 (red).