Figure 9From: Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry Extracellular substrate and metabolite concentrations in pure and mixed cultures. Glutamine, glutamate and glucose were quantified by an enzymatic assay. Gluconate and 2-KDG were quantified by HPLC. Time courses of (A) glutamine (filled symbols) and glutamate (open symbols) and (B) glucose (half-filled symbols), 2-KDG (filled symbols) and gluconate (open symbols) are presented over a cultivation period of 12Â h. For S. aureus neither gluconate nor 2-KDG were detected (n.d., below detection limit). Error bars represent standard deviation of three biological replicates.Back to article page