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Table 5 Sequences of oligonucleotide primers

From: Characterization of 3-phosphoglycerate kinase from Corynebacterium glutamicum and its impact on amino acid production

Name

Sequence (5′-3′)

Function and relevant characteristics

pgk-Cgl-fw

GATCTAGAGAAAGGAG GCCCTTCAGATGGCTGTTAAGACCCTCAAGG

OE of Cgl pgk; start; RBS

pgk-Cgl-fw

GATCTAGATTACTGAGCGAGAATTGCAACG

OE of Cgl pgk; stop;

pgk- pur-fw

CATATG GATGGCTGTTAAGACCCTCAAGG

Purification of Cgl PGK, start; NdeI

pgk- pur-rv

CATATGGATCTAGATTACTGAGCGAGAATTGCAACG

Purification of Cgl PGK, stop; NdeI

pgk-Del-A

GACCTTCAACACCAAGTCTGAG

Del of pgk

pgk-Del-B

CCCATCCACTAAACTTAAACA TGAGGGTCTTAACAGCCATGC

Del of pgk

pgk-Del-C

TGTTTAAGTTTAGTGGATGGG CCCAGGCGTTGCAATTCTC

Del of pgk

pgk-Del-D

CTTCGCAGCAACCAACTCATC

Del of pgk

pgk _Del_ver_fw:

CATACACTGGCGACCAGC

Verification of pgk deletion

pgk _Del_ver_rv

CTGCCTTAACAGAACCACCG

Verification of pgk deletion

  1. Restriction sites are highlighted in bold, linker sequences for crossover PCR and ribosomal binding sites are shown in italics. Abbreviations: OE overexpression, Del deletion, RBS ribosomal binding site, Cgl C. glutamicum.
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BMC Microbiology

ISSN: 1471-2180

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