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Figure 1 | BMC Microbiology

Figure 1

From: Characterization of 3-phosphoglycerate kinase from Corynebacterium glutamicum and its impact on amino acid production

Figure 1

Sodium dodecyl sulfate-polyacrylamide gel electrophoreses of expression and purification of PGK His of C. glutamicum from E. coli BL21 (DE3; pET16b- pgk ). The gel was loaded with cell extract from E. coli (pET16b-pgk) before (lane 1) and 4 h after induction with 0.5 mM IPTG (lane 2), the flow through after Ni-NTA chromatography (lane 3), and the eluate of Ni-NTA chromatography after rebuffering on a PD-10 column (lane 4). Lanes M1 shows protein standards SeaBlue Plus2 prestained standard (Invitrogen) containing protein of the indicated masses.

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BMC Microbiology

ISSN: 1471-2180

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