Effects of voriconazole alone and in combination with cefepime against A. fumigatus monomicrobial and A. fumigatus - P. aeruginosa polymicrobial biofilms as determined by CFU (A) and MTT (B) assays. The biofilms were developed in 24-well cell culture plates and the effectiveness of antimicrobial drug(s) treatment was assessed by the reduction of CFUs and A570 values. Each experiment was performed two different times with the clinical isolates AF53470 and PA56402 using independently prepared conidial suspensions and bacterial cultures, and one time with the laboratory isolates AF36607 and PA27853. Similar results were obtained for both set of isolates. The data were analyzed by two-way ANOVA with Bonferroni post test analysis by comparing each treatment group to the other for statistical significance using Graphpad Prism 5.0. The vertical bar on each data point denotes standard error of the mean for two experiments performed with AF53470 and PA56402. Legends: AF, A. fumigatus monomicrobial biofilm; AF + PA, A. fumigatus-P. aeruginosa polymicrobial biofilm; VCZ, voriconazole; CEF, cefepime.