Table 2 Kinetic parameter values from C. butyricum DSP1 in 6.6 L, 42 L and 150 L bioreactors
From: Scale-up of anaerobic 1,3-propanediol production by Clostridium butyricum DSP1 from crude glycerol
Parameter/fermentation scale | 6.6 L | 42 L | 150 L |
|---|---|---|---|
Time of fermentation (h) | 33 | 32 | 28 |
Max biomass, Xmax (g/L) | 1.2 | 1.2 | 1.3 |
Time taken to reach max biomass, t (h) | 15 | 16 | 14 |
Max specific growth rate, μ (1/h) | 0.067 | 0.062 | 0.071 |
Max 1,3-PD concentration, 1,3-PDmax (g/L) | 37.63 ± 1.2 | 36.40 ± 1.6 | 37.20 ± 1.4 |
1,3-PD productivity P1,3-PD (g/L/h) | 1.12 | 1.13 | 1.33 |
1,3-PD yield, Y1,3-PD (g1,3- PD/gGly) | 0.53 | 0.52 | 0.53 |
Max butyric acid concentration, Butmax (g/L) | 4.26 ± 0.09 | 3.57 ± 0.08 | 4.22 ± 0.07 |
Butyric acid productivity PBut (g/L/h) | 0.13 | 0.11 | 0.15 |
Butyric acid yield, YBut (gBut/gGly) | 0.06 | 0.05 | 0.06 |
Max acetic acid concentration, Acemax (g/L) | 2.0 ± 0.03 | 1.9 ± 0.03 | 2.2 ± 0.02 |
Acetic acid productivity PAce (g/L/h) | 0.06 | 0.06 | 0.08 |
Acetic acid yield, YAce (gLac/gGly) | 0.03 | 0.03 | 0.03 |
Max lactic acid concentration, Lacmax (g/L) | 3.14 ± 0.02 | 2.84 ± 0.03 | 3.63 ± 0.04 |
Lactic acid productivity PLac (g/L/h) | 0.09 | 0.09 | 0.12 |
Lactic acid yield, YLac (gLac/gGly) | 0.04 | 0.04 | 0.05 |