Table 2 Primers used in this study

Primers for mutagenesis of ureA

ureA-UF

5′ GCTCTAGA ATCCTTCATGGGCTGT

Xba I

ureA-UR

5′ GCAGGGAGCGGTGGATAACCTCCATTTG

ureA-DF

5′ TGGAGGTTATCCACCGCTCCCTGCACAC

ureA-DR

5′ CGCATGC TTCCTCATCAGATGGAGCAGACG

Sph I

Inner-ureA-F

5′ TGCATCTCACTCCGCGTGAG

Inner-ureA-R

5′ TACTGGATCGGGGAATGCAC

Primers for mutagenesis of ureC

ureC-UF

5′ TCAGAGCTC CAGGTCGAAGCCGTCTTCAC

Sac I

ureC-UR

5′ GTTTCCGGGTTACCCAGACGGATCTTG

ureC-DF

5′ TCCGTCTGGGTAACCCGGAAACCTTCG

ureC-DR

5′ TCGTCGAC CGTTGGCCACGAAGATGTCC

Sal I

Inner-ureC-F

5′ GTCCGTCCGGAAACCATTGC

Inner-ureC-R

5′ CCTGCGAGGCAAAGGTGATG

Primers for mutagenesis of ureD

ureD-UF

5′ GCGAGCTC CAAGACCGCCATCATCGAAG

Sac I

ureD-UR

5′ GGTACATCAGGTCAAACGCGGCGATGGC

ureD-DF

5′ CGCGTTTGACCTGATGTACCGGGTGGTG

ureD-DR

5′ GCGTCGAC ACCAGATACAGCCACATCAG

Sal I

Inner-ureD-F

5′ TACAGCAGGCGCTGTACTGG

Inner-ureD-R

5′ GCAGCAGCACGTTGGCAAAG

Primers for mutagenesis of ureE

ureE-UF

5′ CGTCTAGA GGAGCCATGTTCCGCGAAT

Xba I

ureE-UR

5′ GCATGGTGATGCAGGGCAATCTCCACT

ureE-DF

5′ AGATTGCCCTGCATCACCATGCGGAAG

ureE-DR

5′ TGCATGC CATCATCGAGGCCAGTCC

Sph I

Inner-ureE-F

5′ TGGCCAGCATCACGCTCAAG

Inner-ureE-R

5′ TGCAGGTGCTGGTGGGTATG

Primers for mutagenesis of arcA1

LPW14961 (arcA1-UF)

5′ CCGCTCGAG TGGATGATCACGGTCAAG

Xho I

LPW14962 (arcA1-UR)

5′ GTATTGCGGTCCTGTTCAACCCAGATCAC

LPW14963 (arcA1-DF)

5′ GGTTGAACAGGACCGCAATACCTATACC

LPW14964 (arcA1-DR)

5′ CTAGTCTAGA TAGCGGGCCAGCTCTTCG

Xba I

LPW16076^b (Inner-arcA1-F)

5′ ACATGCTGACCAGGGTTTG

LPW16077^b (Inner-arcA1-R)

5′ AAAGGCTTGTCGTGCCGTTC

Primers for mutagenesis of arcA2

LPW14965 (arcA2-UF)

5′ CCGCTCGAG GATTTATTCGCCGGAAAC

Xho I

LPW14966 (arcA2-UR)

5′ ACACCGCGATCGTAGTCGTGGTCCTTCTG

LPW14967 (arcA2-DF)

5′ CACGACTACGATCGCGGTGTTGAAGTG

LPW14968 (arcA2-DR)

5′ TGCTCTAGA GTACATGCGGCCCAGAAC

Xba I

LPW16078^c (Inner-arcA2-F)

5′ ATCGCAAGGTGTGCGCCAAC

LPW16079^c (Inner-arcA2-R)

5′ AGCGATTCACGCACCACTTC

Primers for real-time qPCR

LPW21629 (arcA1-F)

5′GTCACCCTCAATCCGATG

LPW21630 (arcA1-R)

5′CACCACACCTTCACCTTG

LPW21631 (arcA2-F)

5′CCGAAGTGGTGCGTGAATC

LPW21632 (arcA2-R)

5′TCTTGTTGGTGTAGGTGTTGC

LPW21635 (rpoB-F)

5′GTGCTGTTCGTCAATGAG

LPW21636 (rpoB-R)

5′TAGGTCGTAGGATTCTTCG

LPW22260 (ureA-F)

5′ATCTATTGCCTCGCCGAAGTG

LPW22261 (ureA-R)

5′AGTGCTGCCGCCGAAATC